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n 2 plus media supplement × 1000  (R&D Systems)


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    R&D Systems n 2 plus media supplement × 1000
    N 2 Plus Media Supplement × 1000, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+2+plus+media+supplement+%C3%97+1000/N-2+Plus+Media+Supplement/pmc06279800__41598_2018_35733_MOESM1_ESM-42-39-54
    Average 95 stars, based on 145 article reviews
    n 2 plus media supplement × 1000 - by Bioz Stars, 2026-09
    95/100 stars

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    Article Title: Zebrafish embryo extracts promote sphere‐forming abilities of human melanoma cell line
    Article Snippet: For growing melanoma spheroid cells, we used DMEM/F12 (Catalog no. 12500‐062; Gibco) medium optimized for growth of neural stem cells, conditioned with glucose 1.55 g/L (Sigma‐Aldrich, St. Louis, MO, USA); L‐Glutamine 73 mg/L (Sigma‐Aldrich); sodium bicarbonate 1.69 g/L (Sigma‐Aldrich); N‐2 Plus Media Supplement ×1000 containing bovine insulin, human transferrin, sodium selenite, putrescine, and progesterone (R&D Systems, Minneapolis, MN, USA); FGF basic 100 ng/mL; EGF 100 ng/mL (both from R&D Systems); penicillin 100 U/mL (Gibco‐Invitrogen); and streptomycin 100 μg/mL (Gibco‐Invitrogen).

    Article Title: Zebrafish embryo extracts promote sphere-forming abilities of human melanoma cell line.
    Article Snippet: Sphere-forming abilities in culture condition are considered a hallmark of cancer stem-like cells, which represents tumor cell invasiveness and stem-like characteristics.. We aimed to show that the sphere-forming subpopulation of human malignant melanoma cell line WM-266-4 acts differently to zebrafish embryo extracts compared with their bulk counterpart.. Spheres were maintained in neural stem cell culture conditions.

    Article Title: Multiple genetic mutations caused by NKX6.3 depletion contribute to gastric tumorigenesis
    Article Snippet: For growing HFE-145 spheroid cells, we used DMEM/F12 (Catalog no. 12500-062; Gibco) medium optimized for growth of neural stem cells, conditioned with glucose 1.55 g/L (Sigma-Aldrich, St. Louis, MO, USA); L-Glutamine 73 mg/L (Sigma-Aldrich); sodium bicarbonate 1.69 g/L (Sigma-Aldrich); N-2 Plus Media Supplement ×1000 containing bovine insulin, human transferrin, sodium selenite, putrescine, and progesterone (R&D Systems, Minneapolis, MN, USA); FGF basic 100 ng/mL; EGF 100 ng/mL (both from R&D Systems); penicillin 100 U/mL (Gibco-Invitrogen); and streptomycin 100 μg/mL (Gibco-Invitrogen).



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    95
    R&D Systems n 2 plus media supplement × 1000
    N 2 Plus Media Supplement × 1000, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+2+plus+media+supplement+%C3%97+1000/N-2+Plus+Media+Supplement/pmc06279800__41598_2018_35733_MOESM1_ESM-42-39-54
    Average 95 stars, based on 1 article reviews
    n 2 plus media supplement × 1000 - by Bioz Stars, 2026-09
    95/100 stars
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